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Biotechnology and Biomedical Engineering Immunology Virtual Lab II Experiments

Aim

Theory

Pretest

Procedure

Simulation

Posttest

References

Feedback

Aim

Theory

Pretest

Procedure

Simulation

Posttest

References

Feedback

Fragmentation of IgG Using Papain

Choose difficulty:

Beginner

Intermediate

Advanced

1.Which is the most abundant antibody present in serum?

a: IgG Explanation

Explanation

b: IgE Explanation

Explanation

c: IgA Explanation

Explanation

d: IgD Explanation

Explanation

2.The enzyme Protease cleaves the IgG antibodies in hinge region and forms 2 fragments. Which of the following are the fragments formed by Papain?

a: F(ab)2 and Fc Explanation

Explanation

b: 2F(ab) and Fc Explanation

Explanation

c: 1F(ab) and 2Fc Explanation

Explanation

d: None of the above Explanation

Explanation

3.Papain digested antibody is able to promote agglutination,percipitation,opsonization etc.

a: True Explanation

Explanation

b: False Explanation

Explanation

4.Which is the chemical used for the further treatment of cleaved fragments of antibody?

a: 70% Methanol Explanation

Explanation

b: Mercaptoethanol Explanation

Explanation

c: Both 1 & 2 Explanation

Explanation

d: None of the above Explanation

Explanation

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