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Biotechnology and Biomedical Engineering Immunology Virtual Lab II Experiments

Aim

Theory

Pretest

Procedure

Simulation

Posttest

References

Feedback

Aim

Theory

Pretest

Procedure

Simulation

Posttest

References

Feedback

Fluorescent Labeling of Antibodies

Choose difficulty:

Beginner

Intermediate

Advanced

1.Antibodies can be labelled with FITC through which of the following residue :

a: Valine Explanation

Explanation

b: Lysine Explanation

Explanation

c: Histidine Explanation

Explanation

d: Glycine Explanation

Explanation

2.Which is the buffer that we used in this experiment for the dialysis of antibodies:

a: PBS Buffer Explanation

Explanation

b: Sodium bicarbonate Buffer Explanation

Explanation

c: Sodium acetate Buffer Explanation

Explanation

d: Sodium citrate Buffer Explanation

Explanation

3.Flourescent Dyes are :

a: Hydrophobic and bind non-covalently to proteins Explanation

Explanation

b: Hydrophobic and bind covalently to proteins Explanation

Explanation

c: Hydrophilic and bind non-covalently to proteins Explanation

Explanation

d: Hydrophilic and covalently to proteins Explanation

Explanation

4.Absorbance used to determine the protein concentration in sample is :

a: 210 nm Explanation

Explanation

b: 280 nm Explanation

Explanation

c: 600 nm Explanation

Explanation

d: 480 nm Explanation

Explanation

5.FITC dye mainly used for labelling which of the following biomolecules : 1. Antigen 2.Antibody 3.Amino acids 4.Lectins

a: 1 only Explanation

Explanation

b: 2 and 3 only Explanation

Explanation

c: 2 and 4 only Explanation

Explanation

d: None of these Explanation

Explanation

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