Immunology Virtual Lab II

1. To introduce the principle of precipitation reactions and differentiate it from agglutination reactions

2. To demonstrate the purification protocols of antibodies for use in research and diagnostics

3. To understand the concepts of mouse Euthanasia. To learn the basic procedures involved in rodent dissection and how to identify and remove lymphoid organs.

4. To understand the basic principles in fragmentation of IgG to a monovalent Fab fragment using Papain digestion. The cleavage of antibodies using the Proteolytic enzyme is first studied by porter in 1959. IgG is cleaved by Papain to give Fab fragments

5. To understand the principles of IgG fragmentation to a divalent F(ab')2 fragment.